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A neuronal lineage marker can be either DNA, m RNA or RNA expressed in a cell of interest.

It can also be a protein tag, as a partial protein, a protein or an epitope that discriminates between different cell types or different states of a common cell.

The next revolutionary technique was invented in 1969 by an American scientist called Joseph G. This technique is called in situ Hybridization and it is used in a large variety of studies but mainly used in developmental biology.

With this technique it is possible to mark some genes expressed in determined areas of the animal.

By immunohistochemistry, anti-Hu stains the nuclei of neurons.

He also described very precisely the purkinje cells, the chick cerebellum and the neuronal circuit of the rodent hippocampus. Albert Coons used for the first time a revolutionary technique that uses the principle of antibodies binding specifically to antigens in the tissues.

He created an immunoflorescent technique for labelling the antibodies.

This technique continues to be widely used in neuroscience studies for identifying different structures.

In 1953 Heinrich Klüver invented a new staining technique called, Luxol Fast Blue stain or LFB, and with this technique it’s possible to detect demyelination in the central nervous system.

Myelin sheath will be stained blue, but other structures will be stained as well.

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